Berera R(1), van Grondelle R, Kennis JT.

The implementation of transient absorption mode in pump—probe microscopy will allow ground-state kinetics to be directly monitored.
1994).The advent of ultrashort tunable laser systems in the early 1990s has opened up a new and … 1999; Van Amerongen and Van Grondelle 2001; Van Grondelle et al. These methods are collectively referred to as pump-and-probe methods although, depending on the time … The principle of ultrafast transient absorption spectroscopy.

Author information: (1)Department of Physics and Astronomy, Faculty of Sciences, VU University Amsterdam, De Boelelaan 1081, Amsterdam, The Netherlands.

Transient absorption spectroscopy, a special type of pump-probe spectroscopy, is a fundamental example of nonlinear spectroscopy.

Transient absorption techniques rely on the use of a probe beam that interrogates the sample before and after excitation with a pulsed laser (pump beam), and monitors absorbance changes either at a selected wavelength (kinetics) or simultaneously at several wavelengths (transient spectra).

The advent of ultrashort tunable laser systems in the 1994).

In transient absorption spectroscopy, a fraction of the molecules is promoted to an electronically excited state by means of an excitation (or pump) pulse.
Depending on the type of experiment, this fraction typically ranges from 0.1% to tens of percents.

1999; Van Amerongen and Van Grondelle 2001; Van Grondelle et al.

Ultrafast transient absorption spectroscopy The principle of ultrafast transient absorption spectroscopy The process of energy transfer in a photosynthetic mem-brane typically takes place on a time scale from less than 100 fs to hundreds of ps (Sundstro¨m et al. Ultrafast transient absorption spectroscopy: principles and application to photosynthetic systems. Transient absorption allows us to follow the evolution of molecular, aggregate or material systems on the ground and excited states as a function of femtosecond to nanosecond time delays. The process of energy transfer in a photosynthetic membrane typically takes place on a time scale from less than 100 fs to hundreds of ps (Sundström et al. The addition of two-photon excitation would improve the microscope spatial resolution as well as making wavelength-dependent spectroscopy possible. A weak probe pulse (i.e., a pulse that has such a low intensity that multiphoton/multistep processes are avoided during probing) is sent through the sample …


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