Genome Editing in Grain Crops . Mice carrying human transgenes or knockin genes may also be useful in the specialized toxicity testing of some pharmaceutical compounds. Moreover, Cas9 genes has been optimized with plant-usage bias codons to …

In this review, we describe the different approaches that can be tested to … The AvrPto gene is under the control of a dexamethasone (DEX)-inducible promoter ().Seeds containing the AvrPto gene were initially provided by Dr Fumiaki Katagiri, University of Minnesota and subsequently also … Interspecies genetic homology of biological targets is generally high enough so that pharmaceutical compounds are active in several species. However, some compounds may exhibit pharmacological activity only against a human target. Precise Gene Editing in Plants. We provide a one-stop KO mouse generation service that covers from sgRNA design and construct, pronuclear microinjection to …

Recently, with the development of the CRISPR/Cas9 gene editing technology, it has become possible to use this technique to generate conditional knockout cell lines.

Our serious scientists and skilled staff have rich theoretical knowledge and practical experience in plant genome editing. Conditional knockout cell lines provide better control of gene expression and provide a more physiologically relevant model for studying the role of target genes and drug discovery.

Efficient gene targeting in higher plants remains challenging. Various CRISPR/Cas9 vector systems were developed for efficient editing of plant genomes, including gene knockout, genomic deletion, disruption of cis-regulatory elements, gene knockin, and suppression of virus infection. Transgenic Arabidopsis thaliana plants were used that conditionally express a bacterial type III effector gene, AvrPto, for suppression of host immunity.

Depending on the excellent platform and experienced scientists, our staff has succeeded in lots of gene knockout projects, ranging from tumor cell line to difficult-to-handle cell line. As a leading-edge platform around the world, we have experienced scientists, skilled staff and remarkable technology system.

NHEJ is imprecise and often generates gene knock-out mutations, whereas HR is precise and leads to gene knockin or replacement when a donor DNA molecule is present. Here, the authors develop a sequential transformation method for CRISPR/Cas9-mediated gene targeting in … In these instances, activity-related … The SpCas9 nuclease is the only Cas to have been used so far to induce gene knock-in in higher plants with efficiencies varying from 0.14 to 36% depending on the species, the delivery strategy and the transformation protocol (see Table 1). In contrast, cucumber plants in which the elF4E gene encoding eukaryotic translation initiation factor 4E was mutated using CRISPR/Cas9 have been tested for resistance against Cucumber vein yellowing virus, Zucchini yellow mosaic virus and Papaya ring spot mosaic virus (Chandrasekaran et … Although homologous recombination (HR)‐mediated gene targeting is widely used in many organisms, including mammals (Thomas and Capecchi, 1987) (Chandrasegaran and Carroll, 2016), the low frequencies of HR in higher plants limit its utilization for gene targeting (Fauser … As a leading provider of gene editing service, CRISPR/Cas9 Platform CB is proud to offer gene knockout cell line generation service with high quality. A novel gene-within-a-gene CRISPR/Cas9 system has been developed that is active in plant cells as well as Chlamydomonas • Low, but useable, rates of gene knockout of target genes can be obtained (e.g., ~1 knockout/10 7 initial Chlamydomonas cells). Gene targeting, that is precise sequence knockin or replacement, is the ultimate goal of precision genome engineering. Hydractinia symbiolongicarpus, a colonial cnidarian, is a tractable model system for many cnidarian-specific and general biological questions. Plant materials. This system along with 80 nucleotide ssDNA supports efficient gene/nucleotide replacement in 2 target genes in C. reinhardtii However, if the knock-out of genes by non-homologous end-joining (NHEJ)-mediated repair of the targeted double-strand breaks (DSBs) induced by the CRISPR-Cas9 system is rather well mastered, the knock-in of genes by homology-driven repair or end-joining remains difficult to perform efficiently in higher plants. With experienced scientists and excellent gene editing platform, our staff has successfully generated various gene KO mice by using CRISPR/Cas9. Various species are available, including but not limited to Arabidopsis, tobacco, B. distachyum and medicago.

CRISPR/Cas9 Platform CB provides clients with professional and comprehensive service of crop genome editing.

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