Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Restriction enzymes: Restriction endonucleases are used to enrich methylated from unmethylated DNA. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site. Isoschizomers enzymes HpaII-MspI and SmaI-XmaI recognize CCGG and CCCGGG, respectively, but HpaII and SmaI lack activity when a methyl group is present in their recognition site [61]. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. The recognition sequence and the cut site usually match, but sometimes the cut site can be dozens of nucleotides away from the recognition site. Isoschizomers and neoschizomers: An isoschizomer is an enzyme that … The classical restriction enzymes cut up, and hence render harmless, any unknown (non-cellular) DNA that enters a bacterial cell as a result of a viral infection. Having supplied restriction enzymes to the research community for over 40 years, NEB has earned the reputation of being the leader in enzyme technologies. Isoschizomers: TspMI, XmaCI, XmaI. In addition, we observe a decrease in alignment upon further digestion and subsequent shortening of the DNA. Cut: Displays the cut site and pattern and products of the cut. Source: Serratia marcescens. Incubation Conditions: Buffer J. Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol. Thermo Scientific FastDigest SmaI restriction enzyme recognizes CCC^GGG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. Some enzymes such as SmaI cut the restriction site exactly in the middle on both strands producing cut DNA products with blunt ends. Isoschizomers and neoschizomers: An isoschizomer is a restriction enzyme that recognizes the Time-Saver™ qualified for digestion in 5-15 minutes Ten enzymes were investigated: seven enzymes with a single cut site (EcoRI, KpnI, NdeI, NotI, NruI, SmaI, XbaI), two enzymes with two cut sites (BstZ17I, EagI), and one enzyme with no cut site (ClaI). Most restriction enzymes cut their corresponding restriction sites in a staggered fashion leaving single-stranded overhangs. 25°C. Cut: Cutting site and DNA products of the cut. Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. (SmaI exhibits 25–50% activity at 37°C.) 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